rnascope multiplex fluorescent detection kit Search Results


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rnascope fluorescent multiplex reagent kit  (Advanced Cell Diagnostics Inc)
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dapi  (Bio-Techne corporation)
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Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
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rnascope multiplex fluorescent reagent kit v2  (Bio-Techne corporation)
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Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
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rnascope fluorescent multiplex (3-plex) reagent kit  (Advanced Cell Diagnostics Inc)
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Advanced Cell Diagnostics Inc rnascope fluorescent multiplex (3-plex) reagent kit
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
Rnascope Fluorescent Multiplex (3 Plex) Reagent Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acc  (Alomone Labs)
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Alomone Labs acc
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
Acc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant dna reagent aav1 syn flex chronos gfp addgene  (Addgene inc)
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Addgene inc recombinant dna reagent aav1 syn flex chronos gfp addgene
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
Recombinant Dna Reagent Aav1 Syn Flex Chronos Gfp Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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drug ici  (Tocris)
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Tocris drug ici
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
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Image Search Results


Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this study counterstained with DAPI nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.

Journal: bioRxiv

Article Title: Identification of novel coloboma candidate genes through conserved gene expression analyses across four vertebrate species

doi: 10.1101/2022.12.22.521600

Figure Lengend Snippet: Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this study counterstained with DAPI nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.

Article Snippet: Slides were then mounted in Fluorsave (Merck #345789) with and counterstained with DAPI (ACD Bio-techne, RNAscope Multiplex Fluorescent Reagent Kit v2 #323100).

Techniques: In Situ, Hybridization, Staining, Expressing